NEP (NC_007364) Virus Tagged ORF Clone

CAT#: VC102426

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein 2 [Influenza A virus (A/Goose/Guangdong/1/96(H5N1))], codon optimized for human cell expression, YP_308672

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102426 represents NCBI reference of YP_308672 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTAACACTATCACCTCATTCCAGGATATTCTGCAGAGAATGAGCAAAATGCAGCTGGAGAGCA
  GTTCAGTTGATCTGAACGGCATGATTACACAGTTCGAACGGCTCAAGATCTACAGGGACTCTCTGGGGGA
  AAGTATGATGCGAATGGGAGACCTCCACAGTTTGCAAAATCGGAATGCTACCTGGAGGAATGAGCTGAGC
  CAAAAATTCGAGGAGATTAGATGGCTCATCGCCGAGTGCAGAAATATACTCACAAAGACAGAGAACAGTT
  TCGAGCAGATTACTTTTCTGCAGGCTTTGCAACTGCTCCTTGAGGTGGAAAGCGAGATACGGACCTTCTC
  TTTCCAGCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102426 representing YP_308672
  Red=Cloning sites Green=Tags
  
  MDSNTITSFQDILQRMSKMQLESSSVDLNGMITQFERLKIYRDSLGESMMRMGDLHSLQNRNATWRNELS
  QKFEEIRWLIAECRNILTKTENSFEQITFLQALQLLLEVESEIRTFSFQLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007364
• ORF Size: 363 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007364.1, YP_308672
• RefSeq ORF: 363
• Locus ID: 3654622
• MW: 14.3 kDa