na (NC_004909) Virus Tagged ORF Clone

CAT#: VC102432

Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza A virus (A/Hong Kong/1073/99(H9N2))], codon optimized for human cell expression, NP_859038

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: na
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102432 represents NCBI reference of NP_859038 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCTAATCAAAAAATTATTGCGCTCGGGAGTGTCTCCATTACGATCGCCACTATCTGTCTCCTGA
  TGCAAATTGCGATACTGGCTACTACCATGACACTGCACTTTAACGAATGTACCAACCCTAGTAATAATCA
  AGCAGTGCCCTGCGAACCTATCATAATCGAGCGGAATATTACAGAAATTGTCCACCTGAACAACACCACC
  ATCGAGAAGGAATCTTGTCCTAAGGTGGCCGAGTACAAGAATTGGAGTAAACCCCAGTGCCAAATCACAG
  GCTTCGCCCCATTTTCCAAAGACAATTCCATTAGACTTTCTGCCGGGGGGGACATCTGGGTAACACGCGA
  GCCCTACGTTAGCTGTGGCCTCGGAAAGTGTTATCAGTTCGCCCTGGGTCAGGGCACCACTCTGAATAAT
  AAACACTCCAACGGGACGATTCACGATCGCTCTCCTCACCGAACGTTGCTCATGAACGAACTGGGCGTTC
  CCTTTCACCTCGGAACCAAGCAGGTGTGCATTGCATGGTCTAGTTCTAGTTGCCATGACGGTAAAGCCTG
  GCTGCATGTTTGTGTGACCGGTGATGATCGAAATGCCACAGCTTCTATCATCTACGACGGAATGCTGACT
  GACAGCATCGGTAGTTGGAGCAAGAACATACTGCGGACTCAGGAATCTGAGTGCGTATGCATCAATGGCA
  CCTGCACGGTGGTAATGACTGACGGCTCAGCCAGTGGGAGAGCCGATACAAAAATTCTTTTTATTCGCGA
  GGGTAAGATTGTGCATATTGGGCCACTTTCCGGCAGCGCCCAACATGTGGAGGAGTGCTCTTGTTACCCT
  CGGTACCCAGAAGTCCGCTGTGTGTGCAGAGACAACTGGAAGGGATCTAACCGACCGGTTCTCTATATAA
  ATGTGGCCGACTACTCCGTCGACAGTTCTTATGTATGTAGCGGGCTGGTGGGCGATACCCCTCGGAACGA
  CGACAGCAGTTCTTCATCAAACTGCAGGGACCCCAACAACGAGCGCGGCGGACCAGGGGTCAAAGGATGG
  GCTTTCGACAACGGCAATGACGTGTGGATGGGTAGAACCATCAAGAAAGATAGTAGGAGTGGATATGAAA
  CATTTAGGGTTGTGGGGGGATGGACCACCGCAAATAGTAAGTCACAGATCAATCGCCAGGTTATCGTGGA
  TTCTGACAATTGGTCAGGATACAGCGGGATCTTTTCCGTGGAAGGAAAGACATGTATTAACCGCTGCTTC
  TATGTGGAGTTGATAAGGGGCAGGCCGCAGGAGACCAGAGTGTGGTGGACATCTAACTCTATAATAGTCT
  TCTGTGGCACCTCCGGGACCTACGGAACAGGTTCATGGCCAGACGGCGCCAACATCAATTTCATGTCAAT
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004909
• ORF Size: 1401 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004909.1, NP_859038
• RefSeq ORF: 1401
• Locus ID: 1460997
• MW: 51.3 kDa