NS1 (NC_004906) Virus Tagged ORF Clone

CAT#: VC102436

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein 1 [Influenza A virus (A/Hong Kong/1073/99(H9N2))], codon optimized for human cell expression, NP_859034


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Reconstitution Protocol

USD 400.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol NS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102436 represents NCBI reference of NP_859034 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCTAACACTGTGAGCAGCTTTCAGGTCGACTGCTTCCTTTGGCACGTGCGCAAGAGATTTGCCG
ACCAGGAGTTGGGAGACGCTCCTTTTCTCGACAGACTTCGGCGCGACCAGAAGAGCCTGAGAGGTCGGGG
CAGTACCCTCGGGCTCGACATCAGGACTGCCACTAGGGAAGGCAAGCACATCGTGGAAAGGATCCTTGAG
GAGGAATCCGACGAGGCCCTGAAGATGACCATCGCCTCTGTCCCAGCAAGCAGGTACCTCACCGAGATGA
CCCTGGAGGAGATGAGCAGGGACTGGCTTATGCTGATCCCGAAACAAAAGGTAACCGGCCCACTGTGCAT
TCGCATGGACCAGGCCGTCATGGGTAAGACTATTATACTTAAAGCCAACTTCTCTGTGATTTTCAATCGG
CTTGAAGCCCTCATTCTGCTGCGCGCCTTTACAGACGAGGGGGCCATCGTCGGTGAAATTTCACCCCTTC
CATCTCTGCCAGGGCACACCGACGAAGATGTGAAAAACGCCATCGGCGTTCTCATCGGGGGGCTTGAATG
GAATGATAACACTGTGAGAGTGTCAGAAACCCTCCAACGATTCACATGGAGGTCTTCAGACGAAAATGGC
CGCAGCCCACTCCCCCCTAAGCAGAAGCGAAAGGTGGAACGGACGATCGAACCTGAGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102436 representing NP_859034
Red=Cloning sites Green=Tags

MDSNTVSSFQVDCFLWHVRKRFADQELGDAPFLDRLRRDQKSLRGRGSTLGLDIRTATREGKHIVERILE
EESDEALKMTIASVPASRYLTEMTLEEMSRDWLMLIPKQKVTGPLCIRMDQAVMGKTIILKANFSVIFNR
LEALILLRAFTDEGAIVGEISPLPSLPGHTDEDVKNAIGVLIGGLEWNDNTVRVSETLQRFTWRSSDENG
RSPLPPKQKRKVERTIEPEV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_004906
ORF Size 690 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_004906.1, NP_859034
RefSeq ORF 690
Locus ID 1460995
MW 26.1 kDa

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