NA (NC_007382) Virus Tagged ORF Clone

CAT#: VC102443

Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza A virus (A/Korea/426/1968(H2N2))], codon optimized for human cell expression, YP_308872

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102443 represents NCBI reference of YP_308872 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCCAACCAAAAGATAATTACAATAGGCTCTGTGTCTCTTACTATCGCCACCGTGTGTTTTCTTA
  TGCAGATCGCCATTCTGGTGACCACAGTCACCCTGCACTTTAAGCAGCACGAATGTGATTCACCCGCCTC
  AAATCAAGTGATGCCATGTGAGCCTATCATAATCGAGCGGAACATTACCGAGATCGTGTACCTGAATAAT
  ACAACTATAGAGAAGGAAATCTGTCCGGAGGTCGTAGAGTACAGAAACTGGTCTAAACCCCAGTGTCAAA
  TAACTGGTTTCGCGCCGTTTAGTAAGGATAACTCCATTAGGTTGTCCGCGGGTGGAGACATTTGGGTTAC
  CCGAGAGCCCTACGTGAGTTGTGACCCCGGCAAATGCTACCAGTTCGCACTTGGACAGGGAACTACTCTG
  GATAATAAACATTCCAACGATACTATTCATGATCGCATTCCGCACCGCACCCTTCTGATGAACGAACTGG
  GTGTCCCATTTCACCTGGGAACCAGGCAGGTTTGTGTAGCATGGTCTAGCAGCTCTTGTCACGATGGGAA
  GGCCTGGCTGCATGTGTGCGTGACTGGTGACGACAAGAATGCTACAGCATCATTTATATACGATGGGCGG
  CTCATGGATTCAATTGGGTCTTGGAGCCAAAATATCCTTAGAACCCAGGAGTCAGAGTGCGTGTGTATCA
  ACGGGACTTGCACCGTGGTAATGACAGATGGTTCCGCATCCGGACGGGCCGACACCCGCATCCTTTTTAT
  CGAAGAAGGCAAGATCGTGCACATTTCCCCCCTGTCAGGCTCCGCGCAGCATGTAGAGGAGTGTAGTTGT
  TACCCAAGGTACCCAGACGTGAGGTGCATTTGCAGAGACAATTGGAAAGGGAGTAACAGACCAGTAATCG
  ATATCAATATGGAGGACTACTCAATCGACTCCTCCTACGTTTGCTCAGGGCTGGTGGGTGACACCCCGAG
  GAATGATGACCGGAGCTCTAACTCAAACTGTCGAAATCCTAACAATGAAAGAGGAAATCCAGGCGTGAAA
  GGCTGGGCCTTCGACAACGGTGACGATGTGTGGATGGGCCGCACGATTTCTAAGGACCTGAGGTCAGGAT
  ACGAAACCTTTAAAGTCATTGGCGGGTGGAGCACTCCCAACAGCAAGTCACAAATTAACCGCCAAGTCAT
  CGTAGACTCCAATAATTGGTCAGGTTATAGCGGAATTTTCAGCGTTGAGGGCAAGCGGTGCATTAACAGA
  TGTTTTTATGTCGAGTTGATCAGGGGCCGGCAGCAGGAAACTCGGGTGTGGTGGACATCTAATAGCATTG
  TTGTGTTCTGCGGCACTTCCGGAACGTACGGTACTGGATCCTGGCCCGATGGAGCCAACATTAATTTTAT
  GCCAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102443 representing YP_308872
  Red=Cloning sites Green=Tags
  
  MNPNQKIITIGSVSLTIATVCFLMQIAILVTTVTLHFKQHECDSPASNQVMPCEPIIIERNITEIVYLNN
  TTIEKEICPEVVEYRNWSKPQCQITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPGKCYQFALGQGTTL
  DNKHSNDTIHDRIPHRTLLMNELGVPFHLGTRQVCVAWSSSSCHDGKAWLHVCVTGDDKNATASFIYDGR
  LMDSIGSWSQNILRTQESECVCINGTCTVVMTDGSASGRADTRILFIEEGKIVHISPLSGSAQHVEECSC
  YPRYPDVRCICRDNWKGSNRPVIDINMEDYSIDSSYVCSGLVGDTPRNDDRSSNSNCRNPNNERGNPGVK
  GWAFDNGDDVWMGRTISKDLRSGYETFKVIGGWSTPNSKSQINRQVIVDSNNWSGYSGIFSVEGKRCINR
  CFYVELIRGRQQETRVWWTSNSIVVFCGTSGTYGTGSWPDGANINFMPI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007382
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007382.1, YP_308872
• RefSeq ORF: 1407
• Locus ID: 3655112
• MW: 52.3 kDa