NS (NC_007380) Virus Tagged ORF Clone

CAT#: VC102452

Myc-DDK-tagged ORF clone of viral ORF for non-structural protein NS1 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308870

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102452 represents NCBI reference of YP_308870 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCAACACCGTGAGCTCCTTTCAAGTCGATTGTTTTCTCTGGCATGTGCGCAAGCAGGTCGTCG
  ATCAAGAACTCGGGGATGCCCCTTTCCTGGATAGGTTGAGGCGCGACCAGAAATCTCTGCGGGGACGGGG
  CTCTACACTCGACCTCGACATTGAGGCCGCGACCCGGGTTGGGAAGCAGATCGTAGAGAGAATCCTGAAA
  GAAGAATCCGATGAAGCTCTGAAGATGACCATGGCCTCCGCCCCTGCTTCCAGGTATCTGACAGATATGA
  CTATTGAAGAGCTCTCTCGCGACTGGTTTATGTTGATGCCGAAGCAGAAAGTGGAGGGGCCGCTTTGCAT
  TAGGATCGACCAAGCGATCATGGACAAAAACATTATGCTGAAGGCTAATTTCTCTGTGATCTTTGACCGC
  CTCGAGACCCTCATTCTCCTGCGGGCCTTTACTGAGGAAGGTGCCATCGTTGGGGAGATCTCACCACTGC
  CCTCTCTCCCTGGGCACACTATCGAAGACGTAAAAAACGCCATCGGCGTGTTGATAGGTGGATTGGAATG
  GAACGACAACACTGTCAGAGTGTCAAAAACGCTTCAAAGATTTGCATGGCGGAGTTCTAACGAAAATGGT
  AGGCCTCCCCTTACCCCCAAGCAGAAGAGGAAGATGGCCCGGACCATCAGATCTAAAGTGCGACGGGATA
  AGATGGCAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102452 representing YP_308870
  Red=Cloning sites Green=Tags
  
  MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQKSLRGRGSTLDLDIEAATRVGKQIVERILK
  EESDEALKMTMASAPASRYLTDMTIEELSRDWFMLMPKQKVEGPLCIRIDQAIMDKNIMLKANFSVIFDR
  LETLILLRAFTEEGAIVGEISPLPSLPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWRSSNENG
  RPPLTPKQKRKMARTIRSKVRRDKMAD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007380
• ORF Size: 711 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007380.1, YP_308870
• RefSeq ORF: 711
• Locus ID: 3655110
• MW: 27.0 kDa