NS (NC_007380) Virus Tagged ORF Clone

CAT#: VC102453

Myc-DDK-tagged ORF clone of viral ORF for non-structural protein NS2 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308869

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102453 represents NCBI reference of YP_308869 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGTAATACCGTCTCTAGCTTCCAGGATATTCTGCTGAGAATGTCCAAGATGCAGCTGGGCTCTA
  GCTCAGAGGACCTCAATGGCATGATCACCCAATTCGAGAGCCTCAAACTGTACCGGGATTCACTGGGTGA
  AGCAGTAATGAGAATGGGAGATTTGCACTCCCTGCAAAACCGAAACGGCAAGTGGCGCGAGCAGTTGGGG
  CAGAAGTTCGAGGAAATTCGCTGGTTGATTGAAGAGGTGAGGCATAGACTCAAAATTACCGAAAACTCCT
  TTGAACAAATTACATTTATGCAGGCACTTCAGCTGCTTTTCGAGGTCGAGCAAGAAATTAGGACATTTTC
  TTTCCAACTTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102453 representing YP_308869
  Red=Cloning sites Green=Tags
  
  MDSNTVSSFQDILLRMSKMQLGSSSEDLNGMITQFESLKLYRDSLGEAVMRMGDLHSLQNRNGKWREQLG
  QKFEEIRWLIEEVRHRLKITENSFEQITFMQALQLLFEVEQEIRTFSFQLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007380
• ORF Size: 363 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007380.1, YP_308869
• RefSeq ORF: 363
• Locus ID: 3655110
• MW: 14.4 kDa