M1 (NC_007367) Virus Tagged ORF Clone

CAT#: VC102458

Myc-DDK-tagged ORF clone of viral ORF for matrix protein 1 [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308841

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102458 represents NCBI reference of YP_308841 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTTCTCACCGAGGTTGAAACATACGTGCTCAGCATAGTGCCTAGTGGCCCGCTGAAGGCCGAGA
  TAGCTCAGAGGCTTGAGGATGTATTCGCCGGCAAAAACACTGATCTGGAAGCCCTGATGGAGTGGTTGAA
  GACACGCCCAATTCTCAGCCCCTTGACCAAGGGCATTCTCGGCTTTGTGTTCACATTGACAGTTCCGTCT
  GAAAGGGGCCTGCAGAGAAGGAGATTCGTACAGAATGCGCTGAACGGGAACGGGGACCCTAACAATATGG
  ATAAGGCTGTTAAACTTTATAGGAAGCTTAAAAGGGAGATCACCTTCCACGGGGCTAAGGAAATTGCATT
  GAGTTACAGCGCTGGCGCCCTGGCAAGCTGCATGGGCCTGATATACAATAGAATGGGCGCGGTGACTACA
  GAGGTGGCCTTTGGCCTGGTGTGTGCTACTTGCGAACAGATAGCAGACTCTCAGCACAGGTCACACCGCC
  AGATGGTGGCAACAACAAATCCACTCATAAAACACGAAAATAGAATGGTTCTGGCATCAACCACCGCCAA
  GGCTATGGAGCAGATGGCCGGTAGCAGCGAACAAGCAGCCGAGGCGATGGAGATAGCATCACAGGCACGG
  CAAATGGTGCAGGCCATGCGCGCCGTGGGCACACACCCAAGCTCAAGCACGGGTTTGAGGGACGACCTCC
  TTGAGAATCTGCAGACATATCAGAAACGCATGGGGGTCCAGATGCAGCGATTCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102458 representing YP_308841
  Red=Cloning sites Green=Tags
  
  MSLLTEVETYVLSIVPSGPLKAEIAQRLEDVFAGKNTDLEALMEWLKTRPILSPLTKGILGFVFTLTVPS
  ERGLQRRRFVQNALNGNGDPNNMDKAVKLYRKLKREITFHGAKEIALSYSAGALASCMGLIYNRMGAVTT
  EVAFGLVCATCEQIADSQHRSHRQMVATTNPLIKHENRMVLASTTAKAMEQMAGSSEQAAEAMEIASQAR
  QMVQAMRAVGTHPSSSTGLRDDLLENLQTYQKRMGVQMQRFK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007367
• ORF Size: 756 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007367.1, YP_308841
• RefSeq ORF: 756
• Locus ID: 3655152
• MW: 27.8 kDa