NA (NC_007368) Virus Tagged ORF Clone

CAT#: VC102460

Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308842

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102460 represents NCBI reference of YP_308842 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCGAATCAGAAAATAATCACAATCGGCAGCGTGAGCTTGACGATTAGCACCATCTGCTTTTTTA
  TGCAGATCGCTATCCTGATTACCACAGTGACGCTGCACTTTAAGCAGTATGAATTCAATTCCCCTCCAAA
  CAACCAGGTGATGCTCTGTGAACCTACTATCATTGAAAGGAATATCACAGAGATCGTGTACTTGACAAAC
  ACCACCATCGAGAAGGAAATGTGCCCTAAACTGGCCGAGTACAGAAACTGGTCCAAGCCCCAGTGTGACA
  TCACCGGGTTCGCACCTTTCTCTAAAGACAATAGCATTCGGTTGTCAGCCGGGGGCGACATCTGGGTGAC
  ACGAGAACCGTATGTCTCTTGCGATCCAGATAAATGTTACCAATTTGCTCTGGGTCAAGGTACAACTCTT
  AACAACGTGCATTCCAATGACACCGTACACGATCGGACCCCTTACCGGACCCTGTTGATGAATGAGTTGG
  GTGTCCCATTTCACCTGGGGACGAAGCAAGTGTGTATCGCCTGGTCATCCTCCTCATGTCACGATGGAAA
  AGCTTGGCTGCACGTTTGCGTGACCGGAGATGATAAGAACGCCACCGCATCATTTATCTACAACGGTCGC
  TTGGTCGACAGCATCGTCAGCTGGAGCAAAAAAATCCTGCGGACACAGGAATCTGAGTGTGTGTGCATCA
  ACGGAACTTGCACGGTAGTTATGACTGACGGCTCTGCGAGTGGGAAGGCTGATACAAAGATCTTGTTCAT
  CGAGGAAGGCAAAATCATCCACACCTCTACCTTGAGCGGGTCTGCGCAGCACGTGGAAGAGTGCAGCTGC
  TACCCGAGATACCCTGGAGTGCGATGTGTGTGTCGCGATAATTGGAAAGGATCTAATAGACCCATTGTCG
  ATATCAATATTAAAGACTACTCTATTGTGTCTTCATATGTGTGTAGCGGGCTGGTGGGGGACACTCCCCG
  AAAAAATGACAGTTCTTCTAGTAGTCATTGTCTGGACCCTAATAATGAGGAAGGTGGCCACGGAGTCAAA
  GGATGGGCTTTTGACGATGGCAATGACGTGTGGATGGGCAGGACGATCTCCGAGAAACTTCGATCCGGCT
  ACGAGACCTTTAAAGTGATCGAGGGTTGGAGTAAACCTAACAGCAAACTTCAGATAAACCGGCAGGTCAT
  CGTGGACCGAGGAAACCGGTCTGGTTACAGCGGCATATTCAGTGTGGAGGGTAAAAGCTGTATCAACAGG
  TGTTTCTATGTAGAACTTATACGAGGTCGCAAAGAAGAAACAGAAGTCTTGTGGACATCTAACTCTATAG
  TCGTCTTCTGTGGGACCTCTGGCACGTACGGGACAGGCTCTTGGCCCGATGGTGCAGACATCAATCTTAT
  GCCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102460 representing YP_308842
  Red=Cloning sites Green=Tags
  
  MNPNQKIITIGSVSLTISTICFFMQIAILITTVTLHFKQYEFNSPPNNQVMLCEPTIIERNITEIVYLTN
  TTIEKEMCPKLAEYRNWSKPQCDITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPDKCYQFALGQGTTL
  NNVHSNDTVHDRTPYRTLLMNELGVPFHLGTKQVCIAWSSSSCHDGKAWLHVCVTGDDKNATASFIYNGR
  LVDSIVSWSKKILRTQESECVCINGTCTVVMTDGSASGKADTKILFIEEGKIIHTSTLSGSAQHVEECSC
  YPRYPGVRCVCRDNWKGSNRPIVDINIKDYSIVSSYVCSGLVGDTPRKNDSSSSSHCLDPNNEEGGHGVK
  GWAFDDGNDVWMGRTISEKLRSGYETFKVIEGWSKPNSKLQINRQVIVDRGNRSGYSGIFSVEGKSCINR
  CFYVELIRGRKEETEVLWTSNSIVVFCGTSGTYGTGSWPDGADINLMPI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007368
• ORF Size: 1407 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007368.1, YP_308842
• RefSeq ORF: 1407
• Locus ID: 3655154
• MW: 52.1 kDa