NS2 (NC_007370) Virus Tagged ORF Clone

CAT#: VC102463

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein 2 [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308844

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102463 represents NCBI reference of YP_308844 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCAAACACCGTAAGTTCCTTCCAAGACATTCTCCTTAGGATGTCAAAAATGCAACTTGGGAGCT
  CATCTGAGGACCTGAACGGGATGATAACACAATTTGAGTCATTGAAAATTTATCGCGACAGCCTTGGTGA
  GGCTGTTATGCGGATGGGTGATCTGCACCTGTTGCAGAATCGAAACGGCAAGTGGAGGGAGCAATTGGGG
  CAGAAGTTCGAGGAGATCAGATGGCTTATTGAGGAGGTGCGACATCGCCTGAAGACTACCGAGAACAGTT
  TCGAACAGATCACCTTCATGCAGGCGTTGCAGCTGCTGTTCGAGGTGGAACAGGAGATCAGAACTTTTTC
  CTTTCAGCTTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102463 representing YP_308844
  Red=Cloning sites Green=Tags
  
  MDSNTVSSFQDILLRMSKMQLGSSSEDLNGMITQFESLKIYRDSLGEAVMRMGDLHLLQNRNGKWREQLG
  QKFEEIRWLIEEVRHRLKTTENSFEQITFMQALQLLFEVEQEIRTFSFQLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007370
• ORF Size: 363 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007370.1, YP_308844
• RefSeq ORF: 363
• Locus ID: 3655157
• MW: 14.4 kDa