NS1 (NC_002020) Virus Tagged ORF Clone

CAT#: VC102470

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS1 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, NP_040984


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Reconstitution Protocol

USD 400.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol NS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102470 represents NCBI reference of NP_040984 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCCAATACAGTGTCAAGCTTTCAAGTGGATTGCTTTCTGTGGCACGTGCGGAAGAGAGTGGCCG
ATCAGGAACTGGGAGACGCCCCTTTTCTGGACCGCCTGAGGCGGGACCAGAAGTCATTGCGGGGGAGGGG
TTCCACGCTGGGTCTGGACATAGAAACGGCGACTAGGGCCGGCAAGCAAATTGTCGAAAGAATTCTCAAG
GAGGAATCCGATGAGGCCCTGAAGATGACAATGGCCTCAGTGCCCGCCTCCCGCTATCTGACCGACATGA
CACTTGAAGAGATGAGTCGCGAGTGGTCCATGCTTATACCGAAGCAAAAGGTCGCAGGTCCGCTGTGCAT
TCGAATGGATCAGGCAATCATGGATAAGAACATCATCCTTAAGGCCAACTTCTCAGTTATTTTTGACAGA
CTGGAGACTCTGATTCTGTTGAGAGCATTTACAGAGGAAGGCGCTATCGTAGGCGAGATATCCCCACTGC
CAAGTCTGCCTGGCCACACTGCTGAAGATGTAAAGAACGCTGTGGGCGTCCTGATTGGCGGATTGGAGTG
GAACGATAATACAGTCCGGGTAAGCGAGACCCTGCAACGCTTCGCGTGGCGGTCTAGTAACGAAAATGGT
CGACCTCCTCTCACCCCCAAGCAAAAGCGGGAGATGGCCGGAACTATCAGGAGCGAAGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102470 representing NP_040984
Red=Cloning sites Green=Tags

MDPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILK
EESDEALKMTMASVPASRYLTDMTLEEMSREWSMLIPKQKVAGPLCIRMDQAIMDKNIILKANFSVIFDR
LETLILLRAFTEEGAIVGEISPLPSLPGHTAEDVKNAVGVLIGGLEWNDNTVRVSETLQRFAWRSSNENG
RPPLTPKQKREMAGTIRSEV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_002020
ORF Size 690 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_002020.1, NP_040984
RefSeq ORF 690
Locus ID 956533
MW 25.9 kDa

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