NS2 (NC_002020) Virus Tagged ORF Clone

CAT#: VC102469

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, NP_040983


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Reconstitution Protocol

USD 400.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol NS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102469 represents NCBI reference of NP_040983 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCAAACACAGTTTCCAGCTTCCAGGATATTCTGCTGCGGATGTCTAAGATGCAGCTTGAATCAT
CTTCCGAAGACCTGAATGGAATGATCACGCAGTTTGAGTCTCTTAAGCTCTACAGGGACTCCTTGGGAGA
AGCTGTCATGAGGATGGGCGATCTGCATAGCCTGCAAAACAGAAATGAGAAATGGCGGGAACAGCTGGGC
CAGAAATTTGAGGAGATACGGTGGTTGATTGAAGAGGTCCGCCACAAGCTTAAAGTGACAGAGAATTCCT
TTGAGCAGATTACTTTCATGCAGGCCCTCCACCTGTTGCTGGAGGTGGAGCAGGAGATCCGGACCTTCAG
TTTCCAGCTCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102469 representing NP_040983
Red=Cloning sites Green=Tags

MDPNTVSSFQDILLRMSKMQLESSSEDLNGMITQFESLKLYRDSLGEAVMRMGDLHSLQNRNEKWREQLG
QKFEEIRWLIEEVRHKLKVTENSFEQITFMQALHLLLEVEQEIRTFSFQLI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_002020
ORF Size 363 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_002020.1, NP_040983
RefSeq ORF 363
Locus ID 956532
MW 14.4 kDa

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