HA (NC_002207) Virus Tagged ORF Clone
CAT#: VC102489
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin [Influenza B virus], codon optimized for human cell expression, NP_056660
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Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC102489 represents NCBI reference of NP_056660 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCAATAATAGTACTCCTTATGGTGGTAACCTCTAACGCGGATAGAATTTGTACCGGGATCACTT CTAGCAACTCACCCCATGTGGTAAAGACCGCAACACAAGGCGAAGTAAACGTCACAGGGGTGATCCCTCT CACCACAACACCCACTAAGAGTCATTTTGCTAATCTGAAAGGGACTCAGACGCGGGGGAAACTCTGTCCC AATTGCTTTAATTGTACAGATCTGGATGTAGCCTTGGGGAGGCCTAAATGTATGGGGAATACCCCTAGCG CAAAGGTATCTATACTGCACGAAGTAAAACCCGCAACTTCTGGGTGCTTTCCTATAATGCACGATCGCAC TAAGATCCGGCAACTTCCAAACCTCCTGCGGGGATACGAGAATATACGGCTCTCCACAAGCAACGTGATT AATACAGAAACTGCCCCTGGCGGCCCTTACAAAGTTGGAACCTCCGGGAGTTGTCCAAATGTGGCGAATG GAAATGGCTTCTTTAACACGATGGCGTGGGTCATTCCAAAGGACAATAACAAAACCGCTATTAATCCTGT GACAGTCGAGGTGCCATATATCTGCTCCGAAGGAGAGGATCAGATAACAGTCTGGGGTTTCCATAGCGAC GATAAGACGCAGATGGAAAGACTGTATGGGGATTCAAATCCCCAGAAGTTTACTTCTAGCGCTAATGGAG TTACTACCCATTATGTAAGCCAGATTGGGGGCTTTCCTAATCAAACCGAAGACGAAGGCCTCAAGCAGTC TGGAAGGATCGTCGTTGACTACATGGTGCAAAAGCCTGGCAAGACAGGCACCATAGTGTATCAGAGAGGA ATCCTCCTCCCCCAGAAGGTCTGGTGCGCTAGCGGAAGGAGTAAGGTTATTAAAGGGAGTCTCCCCCTGA TTGGCGAAGCTGATTGTCTGCATGAGAAATATGGGGGTTTGAATAAGAGTAAGCCATACTACACAGGCGA ACACGCCAAGGCGATCGGGAACTGCCCGATTTGGGTCAAGACTCCGCTGAAGCTGGCCAACGGTACGAAA TACAGGCCTCCAGCAAAACTGCTGAAAGAGAGGGGCTTCTTCGGGGCAATTGCCGGATTCCTGGAAGGCG GGTGGGAAGGTATGATTGCGGGCTGGCATGGCTACACATCTCATGGGGCGCACGGCGTAGCCGTGGCAGC TGACCTTAAGAGTACCCAGGAGGCGATCAACAAGATCACCAAGAACCTGAATTATCTGAGCGAGCTGGAA GTAAAAAACCTGCAGCGATTGAGCGGGGCTATGAATGAGCTCCACGACGAGATTTTGGAGCTTGACGAAA AGGTGGATGATCTTCGGGCGGACACCATCTCCTCCCAGATCGAACTCGCCGTACTGCTGTCTAACGAGGG AATTATTAACTCAGAGGACGAACACCTCCTGGCTTTGGAACGCAAACTGAAGAAAATGCTTGGTCCGTCT GCGGTGGAAATTGGTAATGGGTGCTTCGAGACCAAACATAAATGTAACCAAACCTGTCTGGATCGGATTG CCGCCGGTACCTTTAACGCAGGTGACTTCTCCCTTCCCACCTTCGACAGCCTGAACATCACAGCAGCCAG CCTGAATGACGATGGATTGGACAACCACACCATCTTGCTTTATTATTCCACCGCCGCTTCCTCCCTGGCC GTGACACTCATGATCGCCATCTTCATCGTCTATATGGTGTCAAGGGACAATGTCTCTTGTAGCATTTGCC TT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102489 representing NP_056660
Red=Cloning sites Green=Tags MKAIIVLLMVVTSNADRICTGITSSNSPHVVKTATQGEVNVTGVIPLTTTPTKSHFANLKGTQTRGKLCP NCFNCTDLDVALGRPKCMGNTPSAKVSILHEVKPATSGCFPIMHDRTKIRQLPNLLRGYENIRLSTSNVI NTETAPGGPYKVGTSGSCPNVANGNGFFNTMAWVIPKDNNKTAINPVTVEVPYICSEGEDQITVWGFHSD DKTQMERLYGDSNPQKFTSSANGVTTHYVSQIGGFPNQTEDEGLKQSGRIVVDYMVQKPGKTGTIVYQRG ILLPQKVWCASGRSKVIKGSLPLIGEADCLHEKYGGLNKSKPYYTGEHAKAIGNCPIWVKTPLKLANGTK YRPPAKLLKERGFFGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADLKSTQEAINKITKNLNYLSELE VKNLQRLSGAMNELHDEILELDEKVDDLRADTISSQIELAVLLSNEGIINSEDEHLLALERKLKKMLGPS AVEIGNGCFETKHKCNQTCLDRIAAGTFNAGDFSLPTFDSLNITAASLNDDGLDNHTILLYYSTAASSLA VTLMIAIFIVYMVSRDNVSCSICL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_002207 |
ORF Size | 1752 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Reference Data | |
RefSeq | NC_002207.1, NP_056660 |
RefSeq ORF | 1752 |
Locus ID | 956538 |
MW | 63.3 kDa |
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