PKC eta (PRKCH) (NM_006255) Human Mutant ORF Clone

CAT#: RC402800

PRKCH Mutant (v374I), Myc-DDK-tagged ORF clone of Homo sapiens protein kinase C, eta (PRKCH) as transfection-ready DNA

Specifications

Product Data

• Mutation Description: v374I
• Affected Codon#: 374
• Affected NT#: 1120
• Tag: Myc-DDK
• Effect: Cerebral infarction, association with
• Symbol: PRKCH
• Synonyms: nPKC-eta; PKC-L; PKCL; PRKCL
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_006255
• ORF Size: 2049 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC402800 representing NM_006255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCTGGCACCATGAAGTTCAATGGCTATTTGAGGGTCCGCATCGGTGAGGCAGTGGGGCTGCAGC
  CCACCCGCTGGTCCCTGCGCCACTCGCTCTTCAAGAAGGGCCACCAGCTGCTGGACCCCTATCTGACGGT
  GAGCGTGGACCAGGTGCGCGTGGGCCAGACCAGCACCAAGCAGAAGACCAACAAACCCACGTACAACGAG
  GAGTTTTGCGCTAACGTCACCGACGGCGGCCACCTCGAGTTGGCCGTCTTCCACGAGACGCCCCTGGGCT
  ACGACCACTTCGTGGCCAACTGCACCCTGCAGTTCCAGGAGCTGCTGCGCACGACCGGCGCCTCGGACAC
  CTTCGAGGGTTGGGTGGATCTCGAGCCAGAGGGGAAAGTATTTGTGGTAATAACCCTTACCGGGAGTTTC
  ACTGAAGCTACTCTCCAGAGAGACCGGATCTTCAAACATTTTACCAGGAAGCGCCAAAGGGCTATGCGAA
  GGCGAGTCCACCAGATCAATGGACACAAGTTCATGGCCACGTATCTGAGGCAGCCCACCTACTGCTCTCA
  CTGCAGGGAGTTTATCTGGGGAGTGTTTGGGAAACAGGGTTATCAGTGCCAAGTGTGCACCTGTGTCGTC
  CATAAACGCTGCCATCATCTAATTGTTACAGCCTGTACTTGCCAAAACAATATTAACAAAGTGGATTCAA
  AGATTGCAGAACAGAGGTTCGGGATCAACATCCCACACAAGTTCAGCATCCACAACTACAAAGTGCCAAC
  ATTCTGCGATCACTGTGGCTCACTGCTCTGGGGAATAATGCGACAAGGACTTCAGTGTAAAATATGTAAA
  ATGAATGTGCATATTCGATGTCAAGCGAACGTGGCCCCTAACTGTGGGGTAAATGCGGTGGAACTTGCCA
  AGACCCTGGCAGGGATGGGTCTCCAACCCGGAAATATTTCTCCAACCTCGAAACTCGTTTCCAGATCGAC
  CCTAAGACGACAGGGAAAGGAGAGCAGCAAAGAAGGAAATGGGATTGGGGTTAATTCTTCCAACCGACTT
  GGTATCGACAACTTTGAGTTCATCCGAGTGTTGGGGAAGGGGAGTTTTGGGAAGGTGATGCTTGCAAGAA
  TAAAAGAAACAGGAGACCTCTATGCTGTGAAGGTGCTGAAGAAGGACGTGATTCTGCAGGATGATGATGT
  GGAATGCACCATGACCGAGAAAAGGATCCTGTCTCTGGCCCGCAATCACCCCTTCCTCACTCAGTTGTTC
  TGCTGCTTTCAGACCCCCGATCGTCTGTTTTTTGTGATGGAGTTTGTGAATGGGGGTGACTTGATGTTCC
  ACATTCAGAAGTCTCGTCGTTTTGATGAAGCACGAGCTCGCTTCTATGCTGCAGAAATCATTTCGGCTCT
  CATGTTCCTCCATGATAAAGGAATCATCTATAGAGATCTGAAACTGGACAATGTCCTGTTGGACCACGAG
  GGTCACTGTAAACTGGCAGACTTCGGAATGTGCAAGGAGGGGATTTGCAATGGTGTCACCACGGCCACAT
  TCTGTGGCACGCCAGACTATATCGCTCCAGAGATCCTCCAGGAAATGCTGTACGGGCCTGCAGTAGACTG
  GTGGGCAATGGGCGTGTTGCTCTATGAGATGCTCTGTGGTCACGCGCCTTTTGAGGCAGAGAACGAAGAT
  GACCTCTTTGAGGCCATACTGAATGATGAGGTGGTCTACCCTACCTGGCTCCATGAAGATGCCACAGGGA
  TCCTAAAATCTTTCATGACCAAGAACCCCACCATGCGCTTGGGCAGCCTGACTCAGGGAGGCGAGCACGC
  CATCTTGAGACATCCTTTTTTTAAGGAAATCGACTGGGCCCAGCTGAACCATCGCCAAATAGAACCGCCT
  TTCAGACCCAGAATCAAATCCCGAGAAGATGTCAGTAATTTTGACCCTGACTTCATAAAGGAAGAGCCAG
  TTTTAACTCCAATTGATGAGGGACATCTTCCAATGATTAACCAGGATGAGTTTAGAAACTTTTCCTATGT
  GTCTCCAGAATTGCAACCA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC402800 representing NM_006255
  Red=Cloning site Green=Tags(s)
  
  MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNE
  EFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSF
  TEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVV
  HKRCHHLIVTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICK
  MNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRL
  GIDNFEFIRVLGKGSFGKVMLARIKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLF
  CCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHE
  GHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENED
  DLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNHRQIEPP
  FRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NP_006246
• RefSeq Size: 2049 bp
• RefSeq ORF: 2052 bp
• Locus ID: 5583
• Cytogenetics: 14q23.1
• Domains: C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction, Vascular smooth muscle contraction
• MW: 75.1 kDa
• Gene Summary: 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]'