XRCC1 (NM_006297) Human Mutant ORF Clone

CAT#: RC402803

XRCC1 Mutant (R280H), Myc-DDK-tagged ORF clone of Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1) as transfection-ready DNA

Specifications

Product Data

• Mutation Description: R280H
• Affected Codon#: 280
• Affected NT#: 839
• Tag: Myc-DDK
• Effect: Inresed lun nerisk, ssoiion wih
• Symbol: XRCC1
• Synonyms: RCC; SCAR26
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_006297
• ORF Size: 1899 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC402803 representing NM_006297
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGATCCGCCTCCGCCATGTCGTGTCCTGCAGCAGCCAGGACTCGACTCACTGTGCAGAAAATC
  TTCTCAAGGCAGACACTTACCGAAAATGGCGGGCAGCCAAGGCAGGCGAGAAGACCATCTCTGTGGTCCT
  ACAGTTGGAGAAGGAGGAGCAGATACACAGTGTGGACATTGGGAATGATGGCTCAGCTTTCGTGGAGGTG
  CTGGTGGGCAGTTCAGCTGGAGGCGCTGGGGAGCAAGACTATGAGGTCCTTCTGGTCACCTCATCTTTCA
  TGTCCCCTTCCGAGAGCCGCAGTGGCTCAAACCCCAACCGCGTTCGCATGTTTGGGCCTGACAAGCTGGT
  CCGGGCAGCCGCCGAGAAGCGCTGGGACCGGGTCAAAATTGTTTGCAGCCAGCCCTACAGCAAGGACTCC
  CCCTTTGGCTTGAGTTTTGTACGGTTTCATAGCCCCCCAGACAAAGATGAGGCAGAGGCCCCGTCCCAGA
  AGGTGACAGTGACCAAGCTTGGCCAGTTCCGTGTGAAGGAGGAGGATGAGAGCGCCAACTCTCTGAGGCC
  GGGGGCTCTCTTCTTCAGCCGGATCAACAAGACATCCCCAGTCACAGCCAGCGACCCAGCAGGACCTAGC
  TATGCAGCTGCTACCCTCCAGGCTTCTAGTGCTGCCTCCTCAGCCTCTCCAGTCTCCAGGGCCATAGGCA
  GCACCTCCAAGCCCCAGGAGTCTCCCAAAGGGAAGAGGAAGTTGGATTTGAACCAAGAAGAAAAGAAGAC
  CCCCAGCAAACCACCAGCCCAGCTGTCGCCATCTGTTCCCAAGAGACCTAAATTGCCAGCTCCAACTCAT
  ACCCCAGCCACAGCCCCAGTCCCTGCCCGAGCACAGGGGGCAGTGACAGGCAAACCCCGAGGAGAAGGCA
  CCGAGCCCAGACGACCCCGAGCTGGCCCAGAGGAGCTGGGGAAGATCCTTCAGGGTGTGGTAGTGGTGCT
  GAGTGGCTTCCAGAACCCCTTCCGCTCCGAGCTGCGAGATAAGGCCCTAGAGCTTGGGGCCAAGTATCGG
  CCAGACTGGACCCGGGACAGCACGCACCTCATCTGTGCCTTTGCCAACACCCCCAAGTACAGCCAGGTCC
  TAGGCCTGGGAGGCCGCATCGTGCGTAAGGAGTGGGTGCTGGACTGTCACCGCATGCGTCGGCGGCTGCC
  CTCCCAGAGGTACCTCATGGCAGGGCCAGGTTCCAGCAGTGAGGAGGATGAGGCCTCTCACAGCGGTGGC
  AGCGGAGATGAAGCCCCCAAGCTTCCTCAGAAGCAACCCCAGACCAAAACCAAGCCCACTCAGGCAGCTG
  GACCCAGCTCACCCCAGAAGCCCCCAACCCCTGAAGAGACCAAAGCAGCCTCACCAGTGCTCCAGGAAGA
  TATAGACATTGAGGGGGTACAGTCAGAAGGACAGGACAATGGGGCGGAAGATTCTGGGGACACAGAGGAT
  GAGCTGAGGAGGGTGGCAGAGCAGAAGGAACACAGACTGCCCCCTGGCCAGGAGGAGAATGGGGAAGACC
  CGTATGCAGGCTCCACGGATGAGAACACGGACAGTGAGGAACACCAGGAGCCTCCTGATCTGCCAGTCCC
  TGAGCTCCCAGATTTCTTCCAGGGCAAGCACTTCTTTCTTTACGGGGAGTTCCCTGGGGACGAGCGGCGG
  AAACTCATCCGATACGTCACAGCCTTCAATGGGGAGCTCGAGGACTATATGAGTGACCGGGTTCAGTTTG
  TGATCACAGCACAGGAATGGGATCCCAGCTTTGAGGAGGCCCTGATGGACAACCCCTCCCTGGCATTCGT
  TCGTCCCCGATGGATCTACAGTTGCAATGAGAAGCAGAAGTTACTTCCTCACCAGCTCTATGGGGTGGTG
  CCGCAAGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC402803 representing NM_006297
  Red=Cloning site Green=Tags(s)
  
  MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEV
  LVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDS
  PFGLSFVRFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPS
  YAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLPAPTH
  TPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYR
  PDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGG
  SGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTED
  ELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERR
  KLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVV
  PQA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NP_006288
• RefSeq Size: 1899 bp
• RefSeq ORF: 1902 bp
• Locus ID: 7515
• Cytogenetics: 19q13.31
• Domains: BRCT, XRCC1_N
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• MW: 69.6 kDa
• Gene Summary: 'The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]'