MYO3A (NM_017433) Human Mutant ORF Clone

CAT#: RC403208

  • TrueORF®

MYO3A Mutant (Y1042X), Myc-DDK-tagged ORF clone of Homo sapiens myosin IIIA (MYO3A) as transfection-ready DNA


Reconstitution Protocol

USD 1,760.00

4 Weeks*

Size
    • 10 ug

Product Images

Other products for "MYO3A"

Specifications

Product Data
Mutation Description Y1042X
Affected Codon# 1042
Affected NT# 3126
Tag Myc-DDK
Effect Non-syndromic hearing loss
Symbol MYO3A
Synonyms DFNB30
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Vector pCMV6-Entry
ACCN NM_017433
ORF Size 3123 bp
Sequence Data
>RC403208 representing NM_017433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCCATTAATTGGAAAAACAATCATCTTTGATAACTTTCCTGATCCTTCTGATACATGGGAAATCA
CTGAGACAATTGGCAAAGGAACTTATGGGAAAGTTTTTAAAGTATTGAATAAGAAAAATGGCCAAAAAGC
AGCAGTCAAAATTCTTGATCCAATTCACGATATTGACGAAGAGATTGAAGCAGAATATAACATCTTAAAA
GCACTTTCTGACCACCCTAATGTGGTCAGATTCTATGGGATATACTTTAAGAAGGATAAAGTAAATGGAG
ACAAGCTGTGGTTGGTTCTTGAGCTCTGCAGTGGAGGATCAGTGACTGACCTTGTGAAAGGATTTCTGAA
GAGGGGTGAAAGAATGAGTGAGCCTCTAATTGCCTATATTTTACATGAAGCACTAATGGGACTTCAACAT
TTGCATAACAACAAAACTATCCACAGAGATGTGAAAGGCAATAACATTCTATTGACCACGGAAGGTGGAG
TGAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCACCAGTACCCGGCACCGTCGGAACACATCCGTAGG
AACACCGTTTTGGATGGCTCCTGAGGTGATTGCATGTGAACAGCAATTGGATACCACTTATGACGCCAGA
TGTGACACTTGGTCCCTGGGTATCACGGCCATTGAGCTGGGTGATGGAGATCCTCCACTAGCTGACCTTC
ATCCCATGAGAGCACTCTTCAAAATACCAAGGAATCCACCCCCAAAACTAAGGCAGCCTGAGCTATGGTC
AGCAGAATTCAATGACTTCATAAGCAAGTGCTTGACTAAAGATTATGAAAAGCGTCCAACAGTGTCAGAA
CTTTTACAGCATAAATTCATTACTCAAATTGAGGGCAAAGATGTGATGCTACAAAAACAACTAACGGAAT
TCATTGGCATCCATCAATGCATGGGAGGCACAGAAAAGGCCAGACGTGAACGTATTCACACGAAGAAAGG
GAACTTCAACCGACCTCTAATATCCAATCTGAAGGATGTAGATGATTTAGCAACCCTAGAAATTTTGGAT
GAGAATACAGTCTCAGAGCAACTTGAGAAGTGTTATTCCAGAGATCAGATCTACGTCTATGTGGGAGACA
TACTCATTGCTCTTAACCCTTTTCAGAGTCTGGGTCTTTACTCCACAAAGCATTCCAAACTATATATTGG
ATCAAAGAGAACTGCCAGTCCTCCTCACATTTTTGCAATGGCTGACTTAGGATATCAATCTATGATAACA
TATAATTCAGATCAGTGCATTGTTATTTCTGGAGAAAGTGGTGCTGGAAAGACTGAAAATGCTCATCTTT
TAGTTCAGCAGCTGACAGTGCTTGGAAAGGCTAATAACAGAACCTTGCAAGAGAAGATTTTACAAGTGAA
CAATTTGGTAGAAGCCTTTGGCAATGCCTGCACTATTATAAATGACAATTCTAGCAGATTTGGAAAATAC
TTAGAAATGAAATTCACCTCTTCTGGAGCGGTAGTGGGAGCACAGATTTCTGAATATCTCCTGGAAAAAT
CCCGAGTTATCCACCAAGCTATTGGAGAAAAAAATTTTCATATTTTTTACTACATTTATGCTGGTTTGGC
TGAAAAGAAGAAACTAGCCCATTACAAACTGCCTGAAAATAAGCCTCCCAGGTACCTACAAAATGACCAC
CTCAGAACAGTACAAGACATCATGAATAATAGTTTCTATAAATCCCAGTATGAATTAATTGAGCAATGTT
TCAAAGTCATAGGTTTTACAATGGAGCAACTTGGTAGTATATACAGCATACTCGCTGCAATCTTGAATGT
TGGCAACATTGAATTTTCTTCTGTGGCAACTGAACACCAGATTGACAAGAGCCACATTTCTAATCATACA
GCCCTGGAGAACTGTGCTTCTTTGCTTTGCATTCGGGCAGATGAGCTACAAGAAGCTCTCACCTCCCACT
GTGTGGTCACTAGAGGAGAAACAATTATACGACCCAATACTGTAGAAAAAGCTACCGATGTCAGGGATGC
CATGGCTAAAACTTTATATGGACGTCTCTTTAGTTGGATAGTCAATTGCATTAACAGTTTGTTGAAGCAT
GACTCATCACCAAGTGGGAATGGTGATGAGCTGAGCATTGGCATTCTTGATATATTTGGCTTTGAAAATT
TCAAAAAAAATTCCTTCGAGCAGCTGTGCATTAACATTGCAAATGAACAAATTCAGTATTATTATAATCA
ACATGTGTTTGCATGGGAACAGAATGAATACCTAAATGAAGATGTGGATGCTAGAGTTATTGAATATGAG
GATAACTGGCCCCTCTTAGATATGTTTCTGCAAAAGCCAATGGGTTTACTTTCCCTACTTGATGAAGAAA
GTAGATTTCCCAAGGCCACTGACCAGACTCTTGTAGAAAAATTTGAAGGTAACCTGAAATCACAATACTT
CTGGAGACCCAAAAGAATGGAACTTAGTTTTGGAATTCACCATTATGCAGGAAAGGTCCTCTATAATGCA
AGTGGATTCTTAGCCAAAAACAGAGACACTCTTCCTACTGACATTGTGCTACTTTTGAGGTCATCCGACA
ACAGTGTAATTAGGCAACTAGTCAACCACCCTCTGACCAAAACAGGTAATCTGCCACATTCTAAAACTAA
AAATGTTATAAACTATCAAATGAGGACTTCAGAAAAATTAATCAACCTGGCAAAGGGCGACACTGGAGAA
GCCACACGTCATGCCAGAGAGACAACCAACATGAAAACACAAACGGTTGCATCATATTTTAGATATTCCC
TGATGGATTTGTTGTCTAAAATGGTGGTGGGCCAACCTCATTTTGTCCGTTGCATCAAACCAAATAGTGA
GCGTCAGGCAAGAAAATATGACAAAGAGAAAGTTCTGCTACAGCTTCGGTACACAGGAATTCTGGAAACA
GCAAGAATTCGAAGACTAGGATTCTCCCATCGGATACTTTTTGCTAACTTTATAAAGCGGTACTACCTTC
TCTGCTACAAGTCGAGCGAGGAGCCCCGCATGAGCCCTGACACCTGTGCCACCATTTTGGAAAAAGCTGG
TCTCGATAACTGGGCTCTTGGAAAAACAAAAGTGTTCCTTAAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGA TAAG
GTTTAA
>RC403208 representing NM_017433
Red=Cloning site Green=Tags(s)

MFPLIGKTIIFDNFPDPSDTWEITETIGKGTYGKVFKVLNKKNGQKAAVKILDPIHDIDEEIEAEYNILK
ALSDHPNVVRFYGIYFKKDKVNGDKLWLVLELCSGGSVTDLVKGFLKRGERMSEPLIAYILHEALMGLQH
LHNNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRHRRNTSVGTPFWMAPEVIACEQQLDTTYDAR
CDTWSLGITAIELGDGDPPLADLHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRPTVSE
LLQHKFITQIEGKDVMLQKQLTEFIGIHQCMGGTEKARRERIHTKKGNFNRPLISNLKDVDDLATLEILD
ENTVSEQLEKCYSRDQIYVYVGDILIALNPFQSLGLYSTKHSKLYIGSKRTASPPHIFAMADLGYQSMIT
YNSDQCIVISGESGAGKTENAHLLVQQLTVLGKANNRTLQEKILQVNNLVEAFGNACTIINDNSSRFGKY
LEMKFTSSGAVVGAQISEYLLEKSRVIHQAIGEKNFHIFYYIYAGLAEKKKLAHYKLPENKPPRYLQNDH
LRTVQDIMNNSFYKSQYELIEQCFKVIGFTMEQLGSIYSILAAILNVGNIEFSSVATEHQIDKSHISNHT
ALENCASLLCIRADELQEALTSHCVVTRGETIIRPNTVEKATDVRDAMAKTLYGRLFSWIVNCINSLLKH
DSSPSGNGDELSIGILDIFGFENFKKNSFEQLCINIANEQIQYYYNQHVFAWEQNEYLNEDVDARVIEYE
DNWPLLDMFLQKPMGLLSLLDEESRFPKATDQTLVEKFEGNLKSQYFWRPKRMELSFGIHHYAGKVLYNA
SGFLAKNRDTLPTDIVLLLRSSDNSVIRQLVNHPLTKTGNLPHSKTKNVINYQMRTSEKLINLAKGDTGE
ATRHARETTNMKTQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPNSERQARKYDKEKVLLQLRYTGILET
ARIRRLGFSHRILFANFIKRYYLLCYKSSEEPRMSPDTCATILEKAGLDNWALGKTKVFLK

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NP_059129
RefSeq Size 3123
RefSeq ORF 4851
Locus ID 53904
Protein Families Druggable Genome, Protein Kinase
MW 114.5 kDa
Gene Summary The protein encoded by this gene belongs to the myosin superfamily. Myosins are actin-dependent motor proteins and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as this one, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors. The protein encoded by this gene plays an important role in hearing in humans. Three different recessive, loss of function mutations in the encoded protein have been shown to cause nonsyndromic progressive hearing loss. Expression of this gene is highly restricted, with the strongest expression in retina and cochlea. [provided by RefSeq, Jul 2008]

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.