MYO3A (NM_017433) Human Mutant ORF Clone

CAT#: RC403208

MYO3A Mutant (Y1042X), Myc-DDK-tagged ORF clone of Homo sapiens myosin IIIA (MYO3A) as transfection-ready DNA

Specifications

Product Data

• Mutation Description: Y1042X
• Affected Codon#: 1042
• Affected NT#: 3126
• Tag: Myc-DDK
• Effect: Non-syndromic hearing loss
• Symbol: MYO3A
• Synonyms: DFNB30
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_017433
• ORF Size: 3123 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC403208 representing NM_017433
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCCATTAATTGGAAAAACAATCATCTTTGATAACTTTCCTGATCCTTCTGATACATGGGAAATCA
  CTGAGACAATTGGCAAAGGAACTTATGGGAAAGTTTTTAAAGTATTGAATAAGAAAAATGGCCAAAAAGC
  AGCAGTCAAAATTCTTGATCCAATTCACGATATTGACGAAGAGATTGAAGCAGAATATAACATCTTAAAA
  GCACTTTCTGACCACCCTAATGTGGTCAGATTCTATGGGATATACTTTAAGAAGGATAAAGTAAATGGAG
  ACAAGCTGTGGTTGGTTCTTGAGCTCTGCAGTGGAGGATCAGTGACTGACCTTGTGAAAGGATTTCTGAA
  GAGGGGTGAAAGAATGAGTGAGCCTCTAATTGCCTATATTTTACATGAAGCACTAATGGGACTTCAACAT
  TTGCATAACAACAAAACTATCCACAGAGATGTGAAAGGCAATAACATTCTATTGACCACGGAAGGTGGAG
  TGAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCACCAGTACCCGGCACCGTCGGAACACATCCGTAGG
  AACACCGTTTTGGATGGCTCCTGAGGTGATTGCATGTGAACAGCAATTGGATACCACTTATGACGCCAGA
  TGTGACACTTGGTCCCTGGGTATCACGGCCATTGAGCTGGGTGATGGAGATCCTCCACTAGCTGACCTTC
  ATCCCATGAGAGCACTCTTCAAAATACCAAGGAATCCACCCCCAAAACTAAGGCAGCCTGAGCTATGGTC
  AGCAGAATTCAATGACTTCATAAGCAAGTGCTTGACTAAAGATTATGAAAAGCGTCCAACAGTGTCAGAA
  CTTTTACAGCATAAATTCATTACTCAAATTGAGGGCAAAGATGTGATGCTACAAAAACAACTAACGGAAT
  TCATTGGCATCCATCAATGCATGGGAGGCACAGAAAAGGCCAGACGTGAACGTATTCACACGAAGAAAGG
  GAACTTCAACCGACCTCTAATATCCAATCTGAAGGATGTAGATGATTTAGCAACCCTAGAAATTTTGGAT
  GAGAATACAGTCTCAGAGCAACTTGAGAAGTGTTATTCCAGAGATCAGATCTACGTCTATGTGGGAGACA
  TACTCATTGCTCTTAACCCTTTTCAGAGTCTGGGTCTTTACTCCACAAAGCATTCCAAACTATATATTGG
  ATCAAAGAGAACTGCCAGTCCTCCTCACATTTTTGCAATGGCTGACTTAGGATATCAATCTATGATAACA
  TATAATTCAGATCAGTGCATTGTTATTTCTGGAGAAAGTGGTGCTGGAAAGACTGAAAATGCTCATCTTT
  TAGTTCAGCAGCTGACAGTGCTTGGAAAGGCTAATAACAGAACCTTGCAAGAGAAGATTTTACAAGTGAA
  CAATTTGGTAGAAGCCTTTGGCAATGCCTGCACTATTATAAATGACAATTCTAGCAGATTTGGAAAATAC
  TTAGAAATGAAATTCACCTCTTCTGGAGCGGTAGTGGGAGCACAGATTTCTGAATATCTCCTGGAAAAAT
  CCCGAGTTATCCACCAAGCTATTGGAGAAAAAAATTTTCATATTTTTTACTACATTTATGCTGGTTTGGC
  TGAAAAGAAGAAACTAGCCCATTACAAACTGCCTGAAAATAAGCCTCCCAGGTACCTACAAAATGACCAC
  CTCAGAACAGTACAAGACATCATGAATAATAGTTTCTATAAATCCCAGTATGAATTAATTGAGCAATGTT
  TCAAAGTCATAGGTTTTACAATGGAGCAACTTGGTAGTATATACAGCATACTCGCTGCAATCTTGAATGT
  TGGCAACATTGAATTTTCTTCTGTGGCAACTGAACACCAGATTGACAAGAGCCACATTTCTAATCATACA
  GCCCTGGAGAACTGTGCTTCTTTGCTTTGCATTCGGGCAGATGAGCTACAAGAAGCTCTCACCTCCCACT
  GTGTGGTCACTAGAGGAGAAACAATTATACGACCCAATACTGTAGAAAAAGCTACCGATGTCAGGGATGC
  CATGGCTAAAACTTTATATGGACGTCTCTTTAGTTGGATAGTCAATTGCATTAACAGTTTGTTGAAGCAT
  GACTCATCACCAAGTGGGAATGGTGATGAGCTGAGCATTGGCATTCTTGATATATTTGGCTTTGAAAATT
  TCAAAAAAAATTCCTTCGAGCAGCTGTGCATTAACATTGCAAATGAACAAATTCAGTATTATTATAATCA
  ACATGTGTTTGCATGGGAACAGAATGAATACCTAAATGAAGATGTGGATGCTAGAGTTATTGAATATGAG
  GATAACTGGCCCCTCTTAGATATGTTTCTGCAAAAGCCAATGGGTTTACTTTCCCTACTTGATGAAGAAA
  GTAGATTTCCCAAGGCCACTGACCAGACTCTTGTAGAAAAATTTGAAGGTAACCTGAAATCACAATACTT
  CTGGAGACCCAAAAGAATGGAACTTAGTTTTGGAATTCACCATTATGCAGGAAAGGTCCTCTATAATGCA
  AGTGGATTCTTAGCCAAAAACAGAGACACTCTTCCTACTGACATTGTGCTACTTTTGAGGTCATCCGACA
  ACAGTGTAATTAGGCAACTAGTCAACCACCCTCTGACCAAAACAGGTAATCTGCCACATTCTAAAACTAA
  AAATGTTATAAACTATCAAATGAGGACTTCAGAAAAATTAATCAACCTGGCAAAGGGCGACACTGGAGAA
  GCCACACGTCATGCCAGAGAGACAACCAACATGAAAACACAAACGGTTGCATCATATTTTAGATATTCCC
  TGATGGATTTGTTGTCTAAAATGGTGGTGGGCCAACCTCATTTTGTCCGTTGCATCAAACCAAATAGTGA
  GCGTCAGGCAAGAAAATATGACAAAGAGAAAGTTCTGCTACAGCTTCGGTACACAGGAATTCTGGAAACA
  GCAAGAATTCGAAGACTAGGATTCTCCCATCGGATACTTTTTGCTAACTTTATAAAGCGGTACTACCTTC
  TCTGCTACAAGTCGAGCGAGGAGCCCCGCATGAGCCCTGACACCTGTGCCACCATTTTGGAAAAAGCTGG
  TCTCGATAACTGGGCTCTTGGAAAAACAAAAGTGTTCCTTAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC403208 representing NM_017433
  Red=Cloning site Green=Tags(s)
  
  MFPLIGKTIIFDNFPDPSDTWEITETIGKGTYGKVFKVLNKKNGQKAAVKILDPIHDIDEEIEAEYNILK
  ALSDHPNVVRFYGIYFKKDKVNGDKLWLVLELCSGGSVTDLVKGFLKRGERMSEPLIAYILHEALMGLQH
  LHNNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRHRRNTSVGTPFWMAPEVIACEQQLDTTYDAR
  CDTWSLGITAIELGDGDPPLADLHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRPTVSE
  LLQHKFITQIEGKDVMLQKQLTEFIGIHQCMGGTEKARRERIHTKKGNFNRPLISNLKDVDDLATLEILD
  ENTVSEQLEKCYSRDQIYVYVGDILIALNPFQSLGLYSTKHSKLYIGSKRTASPPHIFAMADLGYQSMIT
  YNSDQCIVISGESGAGKTENAHLLVQQLTVLGKANNRTLQEKILQVNNLVEAFGNACTIINDNSSRFGKY
  LEMKFTSSGAVVGAQISEYLLEKSRVIHQAIGEKNFHIFYYIYAGLAEKKKLAHYKLPENKPPRYLQNDH
  LRTVQDIMNNSFYKSQYELIEQCFKVIGFTMEQLGSIYSILAAILNVGNIEFSSVATEHQIDKSHISNHT
  ALENCASLLCIRADELQEALTSHCVVTRGETIIRPNTVEKATDVRDAMAKTLYGRLFSWIVNCINSLLKH
  DSSPSGNGDELSIGILDIFGFENFKKNSFEQLCINIANEQIQYYYNQHVFAWEQNEYLNEDVDARVIEYE
  DNWPLLDMFLQKPMGLLSLLDEESRFPKATDQTLVEKFEGNLKSQYFWRPKRMELSFGIHHYAGKVLYNA
  SGFLAKNRDTLPTDIVLLLRSSDNSVIRQLVNHPLTKTGNLPHSKTKNVINYQMRTSEKLINLAKGDTGE
  ATRHARETTNMKTQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPNSERQARKYDKEKVLLQLRYTGILET
  ARIRRLGFSHRILFANFIKRYYLLCYKSSEEPRMSPDTCATILEKAGLDNWALGKTKVFLK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NP_059129
• RefSeq Size: 3123
• RefSeq ORF: 4851
• Locus ID: 53904
• Protein Families: Druggable Genome, Protein Kinase
• MW: 114.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the myosin superfamily. Myosins are actin-dependent motor proteins and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as this one, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors. The protein encoded by this gene plays an important role in hearing in humans. Three different recessive, loss of function mutations in the encoded protein have been shown to cause nonsyndromic progressive hearing loss. Expression of this gene is highly restricted, with the strongest expression in retina and cochlea. [provided by RefSeq, Jul 2008]