Human ACAT1 (ACACA) activation kit by CRISPRa

CAT#: GA100023

ACACA CRISPRa kit - CRISPR gene activation of human acetyl-CoA carboxylase alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ACACA
• Locus ID: 31
• Kit Components:

  GA100023G1, ACAT1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100023G2, ACAT1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100023G3, ACAT1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000664, NM_198834, NM_198835, NM_198836, NM_198837, NM_198838, NM_198839
• Synonyms: ACAC; ACC; ACC, ACAC, ACC1, ACCA; ACC-alpha; ACC1; ACCA; acetyl-CoA carboxylase-alpha; acetyl-CoA carboxylase 1; acetyl-Coenzyme A carboxylase alpha; OTTHUMP00000164069
• Summary: 'Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'