Human ATP citrate lyase (ACLY) activation kit by CRISPRa

CAT#: GA100035

ACLY CRISPRa kit - CRISPR gene activation of human ATP citrate lyase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ACLY
• Locus ID: 47
• Kit Components:

  GA100035G1, ATP citrate lyase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100035G2, ATP citrate lyase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100035G3, ATP citrate lyase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001096, NM_001303274, NM_001303275, NM_198830
• Synonyms: ACL; ATPCL; CLATP
• Summary: 'ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]'