Human BAF53A (ACTL6A) activation kit by CRISPRa

CAT#: GA100052

ACTL6A CRISPRa kit - CRISPR gene activation of human actin like 6A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ACTL6A
• Locus ID: 86
• Kit Components:

  GA100052G1, BAF53A gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100052G2, BAF53A gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100052G3, BAF53A gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004301, NM_177989, NM_178042
• Synonyms: ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K
• Summary: 'This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]'