Human APRT activation kit by CRISPRa

CAT#: GA100250

APRT CRISPRa kit - CRISPR gene activation of human adenine phosphoribosyltransferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: APRT
• Locus ID: 353
• Kit Components:

  GA100250G1, APRT gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100250G2, APRT gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100250G3, APRT gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000485, NM_001030018
• Synonyms: AMP; APRTD
• Summary: 'Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'