Human HIF1 beta (ARNT) activation kit by CRISPRa

CAT#: GA100294

ARNT CRISPRa kit - CRISPR gene activation of human aryl hydrocarbon receptor nuclear translocator

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ARNT
• Locus ID: 405
• Kit Components:

  GA100294G1, HIF1 beta gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100294G2, HIF1 beta gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100294G3, HIF1 beta gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001197325, NM_001286035, NM_001286036, NM_001668, NM_178426, NM_178427, NM_001350224, NM_001350225, NM_001350226, NM_001286035.2, NM_001286036.2
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Summary: 'This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]'