Human ATP6V1E1 activation kit by CRISPRa

CAT#: GA100368

ATP6V1E1 CRISPRa kit - CRISPR gene activation of human ATPase H+ transporting V1 subunit E1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ATP6V1E1
• Locus ID: 529
• Kit Components:

  GA100368G1, ATP6V1E1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100368G2, ATP6V1E1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100368G3, ATP6V1E1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001039366, NM_001039367, NM_001696
• Synonyms: ATP6E; ATP6E2; ATP6V1E; P31; Vma4
• Summary: 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]'