Human BARD1 activation kit by CRISPRa

CAT#: GA100399

BARD1 CRISPRa kit - CRISPR gene activation of human BRCA1 associated RING domain 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: BARD1
• Locus ID: 580
• Kit Components:

  GA100399G1, BARD1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100399G2, BARD1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100399G3, BARD1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000465, NM_001282543, NM_001282545, NM_001282548, NM_001282549, NR_104212, NR_104215, NR_104216, NM_001282549.2, NM_001282548.2, NM_001282545.2, NM_001282543.2
• Synonyms: BRCA1 associated RING domain 1; BRCA1-associated RING domain 1; BRCA1-associated RING domain gene 1
• Summary: 'This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]'