Human BAX activation kit by CRISPRa

CAT#: GA100400

BAX CRISPRa kit - CRISPR gene activation of human BCL2 associated X, apoptosis regulator

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: BAX
• Locus ID: 581
• Kit Components:

  GA100400G1, BAX gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100400G2, BAX gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100400G3, BAX gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001291428, NM_001291429, NM_001291430, NM_001291431, NM_004324, NM_138761, NM_138762, NM_138763, NM_138764, NM_138765, NR_027882, NM_001291431.2, NM_001291429.2
• Synonyms: BCL2L4
• Summary: 'The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]'