Human CA8 activation kit by CRISPRa

CAT#: GA100534

CA8 CRISPRa kit - CRISPR gene activation of human carbonic anhydrase 8

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CA8
• Locus ID: 767
• Kit Components:

  GA100534G1, CA8 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100534G2, CA8 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100534G3, CA8 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001321837, NM_001321838, NM_001321839, NM_004056, NR_135821
• Synonyms: CA-RP; CA-VIII; CALS; CAMRQ3; CARP
• Summary: 'The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]'