Human Calpastatin (CAST) activation kit by CRISPRa

CAT#: GA100584

CAST CRISPRa kit - CRISPR gene activation of human calpastatin

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CAST
• Locus ID: 831
• Kit Components:

  GA100584G1, Calpastatin gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100584G2, Calpastatin gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100584G3, Calpastatin gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001042440, NM_001042441, NM_001042442, NM_001042443, NM_001042444, NM_001042445, NM_001042446, NM_001190442, NM_001284212, NM_001284213, NM_001330626, NM_001330627, NM_001330628, NM_001330629, NM_001330630, NM_001330631, NM_001330632, NM_001330633, NM_001330634, NM_001750, NM_173060, NM_173061, NM_173062, NM_173063, NR_033798, NR_104285
• Synonyms: BS-17; PLACK
• Summary: 'The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]'