Human Cyclin D3 (CCND3) activation kit by CRISPRa

CAT#: GA100628

CCND3 CRISPRa kit - CRISPR gene activation of human cyclin D3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CCND3
• Locus ID: 896
• Kit Components:

  GA100628G1, Cyclin D3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100628G2, Cyclin D3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100628G3, Cyclin D3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001136017, NM_001136125, NM_001136126, NM_001287427, NM_001287434, NM_001760
• Synonyms: cyclin D3; D3-type cyclin; G1/S-specific cyclin D3; OTTHUMP00000016390
• Summary: 'The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]'