Human CDC25B activation kit by CRISPRa

CAT#: GA100708

CDC25B CRISPRa kit - CRISPR gene activation of human cell division cycle 25B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CDC25B
• Locus ID: 994
• Kit Components:

  GA100708G1, CDC25B gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100708G2, CDC25B gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100708G3, CDC25B gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001287516, NM_001287517, NM_001287518, NM_001287519, NM_001287520, NM_001287522, NM_001287524, NM_004358, NM_021872, NM_021873, NM_021874, NM_212530, NR_136335, NR_136336
• Summary: 'CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]'