Human CHRNB3 activation kit by CRISPRa

CAT#: GA100819

CHRNB3 CRISPRa kit - CRISPR gene activation of human cholinergic receptor nicotinic beta 3 subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CHRNB3
• Locus ID: 1142
• Kit Components:

  GA100819G1, CHRNB3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100819G2, CHRNB3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100819G3, CHRNB3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000749, NM_001347717
• Synonyms: acetylcholine receptor, neuronal nicotinic, beta-3 subunit; cholinergic receptor, nicotinic, beta 3; cholinergic receptor, nicotinic, beta polypeptide 3
• Summary: 'The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]'