Human Collagen VI (COL6A3) activation kit by CRISPRa

CAT#: GA100907

COL6A3 CRISPRa kit - CRISPR gene activation of human collagen type VI alpha 3 chain

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: COL6A3
• Locus ID: 1293
• Kit Components:

  GA100907G1, Collagen VI gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100907G2, Collagen VI gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100907G3, Collagen VI gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004369, NM_057164, NM_057165, NM_057166, NM_057167
• Synonyms: DKFZp686D23123; DKFZp686K04147; DKFZp686N0262; FLJ34702; FLJ98399
• Summary: 'This gene encodes the alpha-3 chain, one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The alpha-3 chain of type VI collagen is much larger than the alpha-1 and -2 chains. This difference in size is largely due to an increase in the number of subdomains, similar to von Willebrand Factor type A domains, that are found in the amino terminal globular domain of all the alpha chains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in the type VI collagen genes are associated with Bethlem myopathy, a rare autosomal dominant proximal myopathy with early childhood onset. Mutations in this gene are also a cause of Ullrich congenital muscular dystrophy, also referred to as Ullrich scleroatonic muscular dystrophy, an autosomal recessive congenital myopathy that is more severe than Bethlem myopathy. Multiple transcript variants have been identified, but the full-length nature of only some of these variants has been described. [provided by RefSeq, Jun 2009]'