Human Cytochrome P450 1A2 (CYP1A2) activation kit by CRISPRa

CAT#: GA101091

CYP1A2 CRISPRa kit - CRISPR gene activation of human cytochrome P450 family 1 subfamily A member 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CYP1A2
• Locus ID: 1544
• Kit Components:

  GA101091G1, Cytochrome P450 1A2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101091G2, Cytochrome P450 1A2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101091G3, Cytochrome P450 1A2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000761
• Synonyms: CP12; P3-450; P450(PA)
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]'