Human CYP7A1 activation kit by CRISPRa

CAT#: GA101111

CYP7A1 CRISPRa kit - CRISPR gene activation of human cytochrome P450 family 7 subfamily A member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CYP7A1
• Locus ID: 1581
• Kit Components:

  GA101111G1, CYP7A1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101111G2, CYP7A1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101111G3, CYP7A1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000780
• Synonyms: CP7A; CYP7; CYPVII
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]'