Human CYP8B1 activation kit by CRISPRa

CAT#: GA101112

CYP8B1 CRISPRa kit - CRISPR gene activation of human cytochrome P450 family 8 subfamily B member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CYP8B1
• Locus ID: 1582
• Kit Components:

  GA101112G1, CYP8B1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101112G2, CYP8B1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101112G3, CYP8B1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004391
• Synonyms: CP8B; CYP12
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]'