Human DDIT3 activation kit by CRISPRa

CAT#: GA101163

DDIT3 CRISPRa kit - CRISPR gene activation of human DNA damage inducible transcript 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DDIT3
• Locus ID: 1649
• Kit Components:

  GA101163G1, DDIT3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101163G2, DDIT3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101163G3, DDIT3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001195053, NM_001195054, NM_001195055, NM_001195056, NM_001195057, NM_004083
• Synonyms: CEBPZ; CHOP; CHOP-10; CHOP10; GADD153
• Summary: 'This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]'