Human DDX3 (DDX3X) activation kit by CRISPRa

CAT#: GA101167

DDX3X CRISPRa kit - CRISPR gene activation of human DEAD-box helicase 3 X-linked

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DDX3X
• Locus ID: 1654
• Kit Components:

  GA101167G1, DDX3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101167G2, DDX3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101167G3, DDX3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001193416, NM_001193417, NM_001356, NM_024005, NR_126093, NR_126094, NM_001363819
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2
• Summary: 'The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]'