Human DFFB activation kit by CRISPRa

CAT#: GA101187

DFFB CRISPRa kit - CRISPR gene activation of human DNA fragmentation factor subunit beta

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DFFB
• Locus ID: 1677
• Kit Components:

  GA101187G1, DFFB gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101187G2, DFFB gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101187G3, DFFB gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001004285, NM_001004286, NM_001282669, NM_001320132, NM_001320136, NM_004402, NR_104222, NR_135150, NR_135151, NR_135152
• Synonyms: CAD; CPAN; DFF-40; DFF2; DFF40
• Summary: 'Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]'