Human DHFR activation kit by CRISPRa

CAT#: GA101194

DHFR CRISPRa kit - CRISPR gene activation of human dihydrofolate reductase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DHFR
• Locus ID: 1719
• Kit Components:

  GA101194G1, DHFR gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101194G2, DHFR gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101194G3, DHFR gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000791, NM_001290354, NM_001290357, NR_110936
• Synonyms: DHFRP1; DYR
• Summary: 'Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]'