Human Pyruvate Dehydrogenase E2 (DLAT) activation kit by CRISPRa

CAT#: GA101206

DLAT CRISPRa kit - CRISPR gene activation of human dihydrolipoamide S-acetyltransferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DLAT
• Locus ID: 1737
• Kit Components:

  GA101206G1, Pyruvate Dehydrogenase E2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101206G2, Pyruvate Dehydrogenase E2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101206G3, Pyruvate Dehydrogenase E2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001931
• Synonyms: DLTA; PDC-E2; PDCE2
• Summary: 'This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]'