Human Dystrophia myotonica protein kinase (DMPK) activation kit by CRISPRa

CAT#: GA101224

DMPK CRISPRa kit - CRISPR gene activation of human DM1 protein kinase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DMPK
• Locus ID: 1760
• Kit Components:

  GA101224G1, Dystrophia myotonica protein kinase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101224G2, Dystrophia myotonica protein kinase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101224G3, Dystrophia myotonica protein kinase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001081560, NM_001081562, NM_001081563, NM_001288764, NM_001288765, NM_001288766, NM_004409
• Synonyms: DM; DM1; DM1PK; DMK; MDPK; MT-PK
• Summary: 'The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-38 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2016]'