Human DNMT3B activation kit by CRISPRa

CAT#: GA101245

DNMT3B CRISPRa kit - CRISPR gene activation of human DNA methyltransferase 3 beta

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DNMT3B
• Locus ID: 1789
• Kit Components:

  GA101245G1, DNMT3B gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101245G2, DNMT3B gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101245G3, DNMT3B gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001207055, NM_001207056, NM_006892, NM_175848, NM_175849, NM_175850
• Synonyms: ICF; ICF1; M.HsaIIIB
• Summary: 'CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]'