Human elF2 alpha (EIF2S1) activation kit by CRISPRa

CAT#: GA101362

EIF2S1 CRISPRa kit - CRISPR gene activation of human eukaryotic translation initiation factor 2 subunit alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: EIF2S1
• Locus ID: 1965
• Kit Components:

  GA101362G1, elF2 alpha gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101362G2, elF2 alpha gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101362G3, elF2 alpha gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004094
• Synonyms: EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
• Summary: 'The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]'