Human DAP5 (EIF4G2) activation kit by CRISPRa

CAT#: GA101373

EIF4G2 CRISPRa kit - CRISPR gene activation of human eukaryotic translation initiation factor 4 gamma 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: EIF4G2
• Locus ID: 1982
• Kit Components:

  GA101373G1, DAP5 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101373G2, DAP5 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101373G3, DAP5 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001042559, NM_001172705, NM_001418
• Synonyms: AAG1; DAP5; NAT1; P97
• Summary: 'Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]'