Human IRE1 (ERN1) activation kit by CRISPRa

CAT#: GA101455

ERN1 CRISPRa kit - CRISPR gene activation of human endoplasmic reticulum to nucleus signaling 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ERN1
• Locus ID: 2081
• Kit Components:

  GA101455G1, IRE1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101455G2, IRE1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101455G3, IRE1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001433, NM_152461
• Synonyms: hIRE1p; IRE1; IRE1a; IRE1P
• Summary: 'This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]'