Human Estrogen Related Receptor gamma (ESRRG) activation kit by CRISPRa

CAT#: GA101465

ESRRG CRISPRa kit - CRISPR gene activation of human estrogen related receptor gamma

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ESRRG
• Locus ID: 2104
• Kit Components:

  GA101465G1, Estrogen Related Receptor gamma gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101465G2, Estrogen Related Receptor gamma gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101465G3, Estrogen Related Receptor gamma gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001134285, NM_001243505, NM_001243506, NM_001243507, NM_001243509, NM_001243510, NM_001243511, NM_001243512, NM_001243513, NM_001243514, NM_001243515, NM_001243518, NM_001243519, NM_001438, NM_206594, NM_206595, NR_024099, NM_001350122, NM_001350123, NM_001350124, NM_001350125
• Synonyms: ERR3; ERRgamma; NR3B3
• Summary: 'This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region. [provided by RefSeq, Aug 2011]'