Human Factor VIII (F8) activation kit by CRISPRa

CAT#: GA101502

F8 CRISPRa kit - CRISPR gene activation of human coagulation factor VIII

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: F8
• Locus ID: 2157
• Kit Components:

  GA101502G1, Factor VIII gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101502G2, Factor VIII gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101502G3, Factor VIII gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000132, NM_019863
• Synonyms: AHF; DXS1253E; F8B; F8C; FVIII; HEMA
• Summary: 'This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]'