Human FUT3 activation kit by CRISPRa

CAT#: GA101674

FUT3 CRISPRa kit - CRISPR gene activation of human fucosyltransferase 3 (Lewis blood group)

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: FUT3
• Locus ID: 2525
• Kit Components:

  GA101674G1, FUT3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101674G2, FUT3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101674G3, FUT3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000149, NM_001097639, NM_001097640, NM_001097641
• Synonyms: CD174; FT3B; FucT-III; LE; Les
• Summary: 'The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Differences in the expression of this gene are associated with host susceptibility to viral infection. [provided by RefSeq, Aug 2020]'