Human GDNF activation kit by CRISPRa

CAT#: GA101780

GDNF CRISPRa kit - CRISPR gene activation of human glial cell derived neurotrophic factor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: GDNF
• Locus ID: 2668
• Kit Components:

  GA101780G1, GDNF gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101780G2, GDNF gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101780G3, GDNF gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000514, NM_001190468, NM_001190469, NM_001278098, NM_199231, NM_199234
• Synonyms: ATF; ATF1; ATF2; HFB1-GDNF; HSCR3
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]'