Human Histone H2A.X (H2AFX) activation kit by CRISPRa

CAT#: GA102051

H2AFX CRISPRa kit - CRISPR gene activation of human H2A histone family member X

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: H2AX
• Locus ID: 3014
• Kit Components:

  GA102051G1, Histone H2A.X gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102051G2, Histone H2A.X gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102051G3, Histone H2A.X gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002105
• Synonyms: H2A.X; H2A/X; H2AX
• Summary: 'Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]'