Human HLADQA1 (HLA-DQA1) activation kit by CRISPRa

CAT#: GA102127

HLA-DQA1 CRISPRa kit - CRISPR gene activation of human major histocompatibility complex, class II, DQ alpha 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HLA-DQA1
• Locus ID: 3117
• Kit Components:

  GA102127G1, HLADQA1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102127G2, HLADQA1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102127G3, HLADQA1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002122
• Synonyms: CD; CELIAC1; DQ-A1; GSE; HLA-DQA
• Summary: 'HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]'