Human HMGCR activation kit by CRISPRa

CAT#: GA102151

HMGCR CRISPRa kit - CRISPR gene activation of human 3-hydroxy-3-methylglutaryl-CoA reductase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HMGCR
• Locus ID: 3156
• Kit Components:

  GA102151G1, HMGCR gRNA vector 1 in pCas-Guide-CRISPRa

  GA102151G2, HMGCR gRNA vector 2 in pCas-Guide-CRISPRa

  GA102151G3, HMGCR gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000859, NM_001130996, NM_001364187
• Synonyms: LDLCQ3
• Summary: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]