Human HNMT activation kit by CRISPRa

CAT#: GA102167

HNMT CRISPRa kit - CRISPR gene activation of human histamine N-methyltransferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HNMT
• Locus ID: 3176
• Kit Components:

  GA102167G1, HNMT gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102167G2, HNMT gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102167G3, HNMT gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001024074, NM_001024075, NM_006895
• Synonyms: HMT; HNMT-S1; HNMT-S2; MRT51
• Summary: 'In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]'